猪小肠肠粘膜/浆膜/肠衣中的粘多糖组成分析

为了确定污染肝素钠中污染物多硫酸软骨素的来源,将肝素钠的主要原料猪小肠分离为肠粘膜、浆膜、肠衣,采用酶解法提取其中的粘多糖成分,并采用1 HNMR对提取的粘多糖进行定性、定量分析。结果表明,肠粘膜的粘多糖主要成分为肝素钠,并含有硫酸皮肤素和硫酸软骨素C;浆膜中肝素钠和硫酸皮肤素的含量相当,并含有少量硫酸软骨素C;肠衣的粘多糖主要成分为硫酸皮肤素,并含有肝素钠、硫酸软骨素A及硫酸软骨素C;肠粘膜、浆膜、肠衣均不含多硫酸软骨素。

Determination of Glycosaminoglycan Constituents of Intestine Mucosa/Serosa/Casing from Pig Intestine

In order to determine the source of oversulfated chondroitin sulfate（OSCS） in contaminated heparin sodium,the three sections of intestine mucosa,serosa and casing which were isolated from the pig intestine,were digested by trypsin.The products were analyzed by 1HNMR method.The results indicated that principal component of intestine mucosa was heparin sodium,also including dermatan sulfate and chondroitin sulfates C.In the serosa section including little chondroitin sulfates C,the content of heparin sodium was equal to that of dermatan sulfate.The principal component of casing was dermatan sulfate,also including heparin sodium,chondroitin sulfates C and chondroitin sulfates A.OSCS was not discovered in intestine mucosa,serosa and casing.