| 一株高产低温纤维素酶南极细菌的筛选、鉴定及酶学性质初步研究 |
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| 引用本文： | 高丛,缪锦来,郑洲,金青.一株高产低温纤维素酶南极细菌的筛选、鉴定及酶学性质初步研究[J].湖北化工,2012(2):37-39,46. |
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| 作者姓名： | 高丛 缪锦来 郑洲 金青 |
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| 作者单位： | [1]青岛科技大学化工学院,山东青岛266042 [2]国家海洋局第一海洋研究所,山东青岛266061 |
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| 基金项目： | 基金项目：国家自然科学基金资助项目（40876107） |
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| 摘 要： | 从82株南极细菌中筛选出1株高产低温纤维素酶菌株NJ64，通过16SrDNA序列分析对其进行鉴定，并对其产酶条件及酶学性质进行了初步研究。结果表明，NJ64菌株为假交替单胞菌属（Pseudoalteromonassp．）；最适产酶条件为：培养时间72h、初始pH值7．5、培养温度10℃；所产纤维素酶在pH值9．0左右、40℃条件下的酶活性最高，属于低温酶。
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| 关 键 词： | 南极 细菌 低温纤维素酶 |
Screening, Identification and Characterization of A High-Producing Cold-Adapted Cellulase Antarctic Bacterium |
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| GAO Cong,MIAO Jin-lai,ZHENG Zhou,JIN Qing.Screening, Identification and Characterization of A High-Producing Cold-Adapted Cellulase Antarctic Bacterium[J].Hubei Chemical Industry,2012(2):37-39,46. |
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| Authors: | GAO Cong MIAO Jin-lai ZHENG Zhou JIN Qing |
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| Affiliation: | 1. College of Chemical Engineering ,Qingdao University of Science ＆ Technology ,Qingdao 266042,China; 2. First Institute of Oceanography, State Oceanic Administration , Qingdao 266061, China) |
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| Abstract: | A high-producing cold-adapted cellulase antarctic bacterium named NJ64 was isolated from 82 antractic bacteria and identified by 16S rDNA sequence analysis. Its cellulase-producing conditions and enzymatic properties were studied primarily. The strain NJ64 was determined as genus Pseudoalteromonas sp.. The optimum cellulase-producing conditions were obtained as follows：cultural time was 72 h,initial pH value was 7.5, cultural temperature was 10 ℃. The enzymatic activity of cold-adapted cellulase was the highest with pH value of about 9. O,temperature of 40 ℃. |
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| Keywords: | antarctic bacterium cold-adapted cellulase |
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