Accumulation and species distribution of selenium in Se-enriched bacterial cells of the Bifidobacterium animalis 01 |
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Authors: | Bowen Zhang Kang Zhou Jinlan Zhang Qian Chen Guorong Liu Nan Shang Wei Qin Pinglan Li Fengxiang Lin |
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Affiliation: | 1. College of Food Science and Nutritional Engineering, China Agricultural University, 17# Qinghua East Road, Beijing 100083, China;2. Haerbing Meihua Shengwu Technology Co., Ltd., 8-8# Shanghai Road, Heilongjiang 150018, China |
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Abstract: | Bifidobacterium animalis 01 (B. animalis 01) could absorb 16.7–39.6% of inorganic selenium in the medium and transform most of it into organic selenium. Most of the organic selenium (50.7–63.0%) was found in the protein fraction, 9.62–18.7% in the polysaccharide fraction, 0.273–0.754% in the nucleic acid fraction, and 20.8–30.9% in other components. Furthermore, the selenium content of different protein extractions was in the following order: Alkaline-soluble protein-bound Se (46.5–53.4%) > Water-soluble protein-bound Se (27.4–30.8%) > Salt-soluble protein-bound Se (7.79–11.9%) > Alcohol-soluble protein-bound Se (not detected). Additionally, the molecular mass of most proteins or protein subunits containing selenium was about 10–20 kDa. Analysis by LC–MS showed that selenomethionine (SeMet) is the major selenocompound in protein. |
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Keywords: | Se selenium LC liquid chromatography MS mass spectrometry SeMet selenomethionine Se-(Cys)2 selenocystine MRM multiple reactions monitoring |
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