Characterisation of acid-soluble collagen from skin of silver carp (Hypophthalmichthys molitrix) |
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| Authors: | Junjie Zhang Rui Duan Yuanyong Tian Kunihiko Konno |
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| Affiliation: | 1. School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China;2. Faculty of Fisheries Science, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan |
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| Abstract: | Acid-soluble collagen (ASC) from the skin of silver carp (Hypophthalmichthys molitrix) was isolated and some properties of ASC were investigated. SDS–PAGE patterns showed ASC from silver carp skin was type ? collagen. Sulfopropyl-Toyopearl 650(M) column chromatography indicated that ASC from silver carp skin was composed of three kinds of α chains, α1, α2 and α3. Hydroxyproline and proline content of ASC from silver carp skin was 192 residues/1000 residues, which was similar to that of ASC from carp skin. Denaturation temperature (Td) of ASC from silver carp skin was around 29 °C. The results showed that some properties of ASC from silver carp skin were similar to those of ASC from carp skin. However, the peptide map of ASC from silver carp skin digested by pepsin was distinguished with that of ASC from carp skin. |
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| Keywords: | Silver carp ASC Chromatography Denaturation temperature Peptide map |
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