SYBRGreenER™ Real-Time PCR to detect almond in traces in processed food |
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Authors: | Simona Pafundo Mariolina GullìNelson Marmiroli |
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Affiliation: | Division of Genetics and Environmental Biotechnology, Department of Environmental Sciences, University of Parma, Viale G.P. Usberti 11/A, 43100 Parma, Italy |
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Abstract: | Because food ingredients are sometimes considered as causative factors in IgE mediated food allergies, DNA-based tests may prove to be very useful to establish whether allergenic species have been used in foodstuffs production. The development of two SYBR®GreenER™ Real-Time PCR assays, targeting Pru 1 and rbcL genes, based on melting curve analysis, to detect allergen species in food has been presented. Applicability of these methods was assessed with several commercial products containing processed almond. |
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Keywords: | Almond allergen SYBR® GreenER&trade Molecular marker Real-Time PCR |
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