Acidic degradation and enhanced antioxidant activities of exopolysaccharides from Cordyceps sinensis mycelial culture |
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| Authors: | Jing Kun Yan Lin Li Zhao Mei Wang Po Hong Leung Wen Qiang Wang Jian Yong Wu |
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| Affiliation: | 1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China;2. Department of Applied Biology and Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China |
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| Abstract: | An exopolysaccharide (EPS) fraction isolated from mycelial culture of a Cordyceps sinensis fungus, designated EPS-1 with an average molecular weight (MW) of 38 kDa, was hydrolysed in dilute sulphuric acid solution at pH 1 and 90 °C, yielding two major MW fractions, 3.0 kDa and 30 kDa, respectively. While the proportion of lower MW fraction increased with the hydrolysis period (18% in 0.5 h and 92% in 10 h), the polydispersity (Mw/Mn) of EPS decreased steadily (from 1.47 initially to 1.10 in 10 h). The IR spectra of hydrolysed EPS fractions showed changes only in the C–O–C and C–O–H band peaks from that of EPS-1. These results suggest that the hydrolysis of EPS in the acidic solution caused the EPS degradation mainly by cleaving the glycosidic linkage but no change in the primary molecular structure. The hydrolysed EPS fractions had much higher (30–80%) antioxidant and radical-scavenging activities. |
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| Keywords: | Medicinal fungus Polysaccharides Hydrolysis Molecular weight Antioxidant activity Radical scavenging |
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