Antioxidant activity and genotoxic effect on HeLa cells of phenolic compounds from infusions of Quercus resinosa leaves |
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| Authors: | Nuria Elizabeth Rocha-Guzmá n,José Alberto Gallegos-Infante,Rubé n Francisco Gonzá lez-Laredo,Rosalí a Reynoso-Camacho,Minerva Ramos-Gó mez,Teresa Garcia-Gasca,M. Eustolia Rodrí guez-Muñ oz,Salvador Horacio Guzmá n-Maldonado,Luis Medina-Torres,Blanca Azalia Lujan-Garcí a |
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| Affiliation: | 1. Instituto Tecnológico de Durango, Departamento de Ings., Química y Bioquímica, Blvd. Felipe Pescador 1830 Ote., 34080 Durango, Dgo., Mexico;2. Universidad Autónoma de Querétaro, Facultad de Química, Depto. de Investigación y Posgrado en Alimentos (DIPA), Centro Universitario, Cerro de las Campanas s/n, Querétaro, Qro., Mexico;3. Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Av. de la ciencia S/N, Juriquilla, Qro., Mexico;4. Universidad Autónoma de Querétaro, Facultad de Química, CEACA, Centro Universitario, Cerro de las campanas s/n, Querétaro, Qro., Mexico;5. Unidad de Biotecnología, Campo Experimental Bajío, Centro de Investigación Regional del Centro, INIFAP, Km 6. Carr., Celaya – San Miguel Allende AP 112, Celaya, Gto., Mexico;6. Departamento de Ingeniería Química, Conjunto “E”, Facultad de Química, UNAM, Cd. Universitaria, 04510 México, DF, Mexico |
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| Abstract: | Water infusions of mature and fresh Quercus resinosa leaves were evaluated for antioxidant activity and genotoxic effects on HeLa cells. Native Mexicans used to drink Q. resinosa leaves tea as a refreshing beverage. The air dried leaves were pulverised and boiled in water, then their phenolic content and condensed tannins were determined. The chromatographic profile of 15 phenolic components in Quercus leaves infusions was also determined by HPLC. In vitro analysis of antioxidant capacity of leaves infusion extracts were performed by the DPPH method and the deoxyribose assay. The genotoxicity of Q. resinosa leaves extracts was evaluated on HeLa cells as well as its underlying mechanism by the single-cell electrophoresis assay (comet assay). Results show that fresh leaves infusions increase the oxidative process and other damage to DNA in transformed human cells. Fresh leaves from Q. resinosa may serve as a potential source of phenolics with anticancer activity. |
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| Keywords: | Antioxidants Phenolics Quercus HeLa cells |
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