Fluorescence anisotropy analysis of protein–antibody interaction |
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| Authors: | Nadia Barbero Lucia Napione Pierluigi Quagliotto Simona Pavan Claudia Barolo Ermanno Barni Federico Bussolino Guido Viscardi |
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| Affiliation: | 1. Institute of Material Science, Vietnam Academy of Science and Technology, 18, Hoang Quoc Viet Road, Cau Giay, Hanoi, Viet Nam;2. Research Center for Environmental Technology and Sustainable Development, Hanoi University of Science, Hanoi, Viet Nam;3. School of Chemical Engineering, Hanoi University of Science and Technology, 1, Dai Co Viet Road, Hanoi, Viet Nam;4. Department of Chemistry, Hanyang University, Seoul 133-791, Republic of Korea |
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| Abstract: | The interaction between glutathione S-transferase and its antibody α-glutathione S-transferase (B-14) was studied using fluorescence anisotropy, subsequent to glutathione S-transferase bioconjugation with fluorescein-5-maleimide, leading to the determination of the dissociation and association binding constants, Kd and Ka; good binding specificity was observed between glutathione S-transferase and the antibody B-14. The use of spectroscopic techniques, fluorescence anisotropy in particular, is a useful and favourable tool to study biochemical problems. |
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