A fluorescein derivative FLTC as a chemosensor for Hg2 + and Ag+ and its application in living-cell imaging |
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| Affiliation: | 1. Jiangsu Key Laboratory of Pesticide Science, Department of Science, Nanjing Agricultural University, 1 Tongwei Road, Nanjing 210095, PR China;2. School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, PR China;3. School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China;4. Department of Biochemical Engineering, Nanjing University of Science and Technology, 200 Xiaolinwei, Nanjing 210094, PR China |
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| Abstract: | FLTC was synthesized and used as a fluorescent chemosensor to detect Hg2 +. It showed high selectivity toward Hg2 + over many heavy metal ions in an ethanol–H2O (3:2, v/v, HEPES buffer, 0.5 mM, pH 7.15) solution with a detection limit of 0.21 μM. After complexation with Hg2 +, FLTC showed extremely high selectivity toward Ag+ with a detection limit of 0.009 μM. Therefore, detection of Hg2 + and Ag+ could be realized using FLTC and the FLTC–Hg2 + complex, respectively. Cytotoxicity assays and fluorescence microscopy analysis showed that FLTC could be used as a fluorescent probe to detect Hg2 + and Ag+ in L-02 human liver cells. |
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