| 以UPS构建的重组酿酒酵母表达载体及其稳定性研究 |
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| 引用本文： | 王弘,郑文岭,杨太成,冼江. 以UPS构建的重组酿酒酵母表达载体及其稳定性研究[J]. 食品与发酵工业, 2005, 31(8): 4-7 |
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| 作者姓名： | 王弘 郑文岭 杨太成 冼江 |
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| 作者单位： | 1. 华南农业大学食品学院,广州,510640
2. 广州军区广州总医院医学试验科,广州,510010 |
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| 基金项目： | 国家自然科学基金(No.30400354)，广东自然科学基金(No.04300502) |
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| 摘 要： | 采用通用载体质粒融合系统UPS构建了一种以绿色荧光蛋白GFP为报告基因的酿酒酵母表达载体pYES-GFP,并研究了其在重组酿酒酵母中的稳定性。实验表明,所构建的附加型质粒pYES-GFP在经近100代传代后,仍未发生丢失,具有良好的稳定性。同时,通过实验验证了,UPS在进行表达载体构建时快速、简便、高效。
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| 关 键 词： | 酿酒酵母 表达载体 GFP基因 UPS |
| 修稿时间： | 2005-01-06 |
Study on Construction and Stability of Saccharomyces cerevisiae Expression Plasmid by UPS |
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| Wang Hong,Zhen Wenling,Yang Taicheng,Xian Jiang. Study on Construction and Stability of Saccharomyces cerevisiae Expression Plasmid by UPS[J]. Food and Fermentation Industries, 2005, 31(8): 4-7 |
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| Authors: | Wang Hong Zhen Wenling Yang Taicheng Xian Jiang |
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| Affiliation: | Wang Hong1 Zhen Wenling2 Yang Taicheng2 Xian Jiang2 1 |
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| Abstract: | By univector plasmid fusion system (UPS), a yeast expression plasmid pYES-GFP was constructed with green fluorescent protein (GFP) as report gene. The results demonstrated that after over 100 passages, recombinant strain still kept stable plasmid inheritance and consistence. At the same time,high efficiency and convenience of UPS was also testified. |
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| Keywords: | Saccharomyces cerevisiae expression vector GFP gene univector plasmid fusion system(UPS) |
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