| 丹酰氨基酸的胶束毛细管电动色谱分离方法研究 |
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| 引用本文： | 张莹,黄志东,内仓和雄. 丹酰氨基酸的胶束毛细管电动色谱分离方法研究[J]. 现代仪器, 2001, 0(1): 42-44 |
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| 作者姓名： | 张莹 黄志东 内仓和雄 |
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| 作者单位： | 天津市药品检验所,天津,300070 |
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| 摘 要： | 本文用高效毛细管电泳法对9种混合丹酰氨基酸的分离条件进行了探讨。实验条件为:运行缓冲液20mM硼酸钠缓冲液,内含130mM的十二烷基硫酸钠(SDS),pH9.1;工作电压:22kV;电流:146μA;检测波长:214nm;测定温度:20℃;以气压方式进样3s,9种丹酰氨基酸获得了完全分离。
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| 关 键 词： | 高效毛细管电泳法 丹酰氨基酸 |
Study on Determination of DNS-AAs by Micellar Electrokinetic Chromatography |
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| Zhang Ying Huang Zhidong KAZUO UCHKURA. Study on Determination of DNS-AAs by Micellar Electrokinetic Chromatography[J]. Modern Instruments, 2001, 0(1): 42-44 |
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| Authors: | Zhang Ying Huang Zhidong KAZUO UCHKURA |
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| Affiliation: | Tianjin municipal institute for drug control Tianjin 300070 |
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| Abstract: | A method has been developed for the determination of DNS-AAs by Micellar Electrokinetic Chromatogra-phy.The measurements were carried out at 20℃ and in a buffer containing 20mmol/L Sodium borate, 130mmol/L SDS at pH9.1, at 22kV running voltage applied to a 55cm× 75μm i. d. (50cm effective length) untreated fused-sili-ca capillary.Samples were introduced atmospherically with an injection time of 3s.The 9 DNS-AAs were determined by a UV detector at 214nm.The 9 DNS-AAs were separated completely. |
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| Keywords: | Micellar Electrokinetic Chromatpgraphy DNA-AAs |
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