2450MHz微波与γ射线联合辐射对大鼠神经胶质细胞的影响

探讨微波及微波联合γ射线照射对大鼠神经胶质细胞凋亡率、线粒体膜电位(MMP)、胞内游离Ca2+和Ca2+-Mg2+-ATP酶活性的影响。将原代神经胶质细胞分为对照组(C)、微波组(M)、电离组(I)和联合组(IM)。M和IM组接受4mW/cm2的微波辐射3d,2h/d;第4天,I和IM组接受5Gy1Gy/min)的γ射线照射。采用Ca2+-Mg2+-ATP酶测试盒检测胞内Ca2+-Mg2+-ATP酶;流式细胞术检测细胞凋亡和MMP;流式细胞术和共聚焦激光扫描显微镜检测胞内游离Ca2+变化。结果发现,与C组相比,I和IM组细胞的凋亡率显著升高,各组细胞MMP未见明显变化;M、I和IM组胞内游离Ca2+明显升高,Ca2+-Mg2+-ATP酶活性显著降低,差异均有统计学意义(p0.05)。结果提示,在本试验条件下,2450MHz微波可使胞内Ca2+显著上升,Ca2+-Mg2+-ATP酶活性显著降低;未观察到微波与γ射线照射大鼠神经胶质细胞的联合作用。

Biological effects of 2450 MHz microwave combined with γ-rays on rat cultured gliacytes

To explore the biological effects of microwave combined with γ-rays on apoptosis, mitochondrion membrane potential（MMP）, Ca2＋and Ca2＋-Mg2＋-ATPase in rat cultured gliacytes, primarily cultured gliacytes were randomly divided into the control group（C）, the microwave group（M）, the y-rays group（I） and the combined group （IM）. The M and IM groups were exposed to 4 mW/cm2 microwave 2h/d for 3 days. The I and IM groups were given 5 Gy 60Co γ-rays on the forth day. Ca2＋-Mg2＋-ATPase was detected by kit. Apoptosis, MMP and Ca2＋ were detected by flow cytometry（FCM）. The results show that the ratio of apoptosis of the I and IM groups were significantly in- creased, and the contents of Ca2＋ in the cytoplasm of M, I and IM group cells were all significantly higher meanwhile CaZ＋-Mg2＋-ATPase activity was lower than that of the control group; but MMP had no obvious changes in the four groups. Under the experimental conditions, the microwave radiation did not induce evident changes in MMP. How- ever, the content of Ca2＋ in the cytoplasm increased significantly and Ca2＋-Mg2＋-ATPase decreased obviously. No synergic effects were observed in the treatment with microwave before exposure to y-rays.