Enhancement of hydrogen production rate by high biomass concentrations of Thermotoga neapolitana |
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Authors: | Gilbert Dreschke Giuliana d’Ippolito Antonio Panico Piet N.L. Lens Giovanni Esposito Angelo Fontana |
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Affiliation: | 1. Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, Via Di Biasio, 43, 03043, Cassino, FR, Italy;2. Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry, Italian National Council of Research, Via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy;3. Telematic University Pegaso, Piazza Trieste e Trento, 48, 80132, Naples, Italy;4. UNESCO – IHE Institute for Water Education, Westvest 7, 2611-AX Delft, The Netherlands |
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Abstract: | The objective of this study was to enhance the hydrogen production rate of dark fermentation in batch operation. For the first time, the hyperthermophilic pure culture of Thermotoga neapolitana cf. Capnolactica was applied at elevated biomass concentrations. The increase of the initial biomass concentration from 0.46 to 1.74 g cell dry weight/L led to a general acceleration of the fermentation process, reducing the fermentation time of 5 g glucose/L down to 3 h with a lag phase of 0.4 h. The volumetric hydrogen production rate increased from 323 (±11) to 654 (±30) mL/L/h with a concomitant enhancement of the biomass growth and glucose consumption rate. The hydrogen yield of 2.45 (±0.09) mol H2/mol glucose, the hydrogen concentration of 68% in the produced gas and the composition of the end products in the digestate, i.e. 62.3 (±2.5)% acetic acid, 23.5 (±2.9)% lactic acid and 2.3 (±0.1)% alanine, remained unaffected at increasing biomass concentrations. |
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Keywords: | Hydrogen Biomass concentration Dark fermentation Hyperthermophilic CDW Cell dry weight [g/L] HPR Hydrogen production rate AA Acetic acid LA Lactic acid |
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