The expressional level of tankyrase-1 gene and its regulation in colorectal cancer in a Saudi population

Tankyrase1 plays an essential role in cancer progression by regulating telomere length. The study aimedto determine expression of TNKS1 and its regulation in colorectal cancer (CRC) in 20 samples from Saudi patients.mRNA expression of TNKS1 in CRC and paired normal tissues was measured by qRT-PCR. Epigenetic modificationof TNKS1 promoter was determined by methylation-specific PCR while somatic mutation was analyzed by Sangersequencing in exon 10 of the gene. All cancerous and normal tissues expressed TNKS1, but level of expression in CRCtissues was significantly associated with tumor stage though no other parameters; age, gender, and tumor location,showed any correlation. Expression of TNKS1 was markedly higher in earlier (I, II) than later (III, IV) stages of CRCdevelopment. Both cancerous and healthy tissues had unmethylated promoter. Sanger sequencing of exon 10 maskedany somatic mutation in the samples. Our findings suggest that up-regulation of TNKS1 was inversely correlated withcancer progression in CRC, indicating that TNKS1 participates in the initiation of CRC by stabilizing telomere length inthe first phase of cancer progression. Mechanisms other than TNKS1 might play a role in malignant tumor progressionand telomere maintenance in the late stages of CRC.