克鲁维酵母突变株UV-G-40-3菊粉酶性质的研究

研究了克鲁维酵母突变株（Ｋｌｕｙｖｅｒｏｍｙｃｅｓ－ＵＶ－Ｇ－４０－３）所产菊粉酶的分布为胞外酶∶胞壁酶∶胞内酶比是５．７∶１．６∶１。该酶Ｓ／Ｉ为５．３，最适温度为５０℃，最适ｐＨ为４．５，在５０℃以下、ｐＨ４．５～８的范围内比较稳定，４℃贮存稳定性好，１４ｄ后仍保持７６％活力，为外切型菊粉酶，酶解粗菊糖（洋姜提取液）活性为纯菊糖的４倍。

Study on Characteristics of Inulinase by Kluyveromyces UV-G-40-3

In this paper, the distribution of inulinase which produced by Kluyveromyces UVG403 is discussed and the inulimase's ratio of extracell, cell wall and intracell was about 5.71.61. The S/I ratio (relative activity with sucrose and inulin) of that enzyme was 5.3, The optimal pH and temperature for the enzyme activity was 4.5 and 50 respectively. The enzyme was stable at pH 4.58 and 50, put the enzyme at 4 for 14 days, 75% of the enzyme activity remained,. The inulinase activity showed an crude inulin extract from Jerusalem artictoke was 4 times as much as that of inulin.