可德兰寡糖的制备及其组分分析

目的：可德兰多糖是目前发现的唯一不含分支的线性β-1,3- 葡聚糖，由于难溶于水而限制其生物活性的研究，为此对可德兰寡糖的制备及其组分进行研究。方法：采用化学法酸解可德兰多糖。结果：通过红外光谱与基质辅助激光解析/ 电离飞行时间质谱(MALDI-TOF MS)的检测，结果表明：可德兰多糖降解后结构未发生变 化，降解产物的聚合度为2～19，其中聚合度为5 的成分含量最高；高效阴离子交换色谱脉冲安培检测法分析结果表明：反应温度105℃、反应时间150～180min 是降解可德兰寡糖的合理反应条件，降解效果最佳。结论：本研究建立的方法可简单、高效的降解可德兰多糖，制备其寡糖。

Preparation and Component Analysis of Curdlan Oligomers

Beta-1,3-glucan possesses extensive application and application values due to diverse biological activity. Curdlan, an extracellular bacterial polysaccharide, is a linear beta-1,3-glucan, and its water insolubility significantly limits its application. The aim of this study was to prepare the soluble curdlan oligomers (CRDO) for biological activities assay. In the homogeneous solution, CRDO structure change by acid hydrolysis and the influence of different factors on the degradation of curdlan measured by HPAEC-PAD was investigated. Compared with curdlan, the structure of CRDO was not changed measured by FTIR spectrometer and MALDI-TOF MS, and the degree of polymerization (DP) of CRDO obtained was 2 － 19 with the most abundance of DP 5. The reaction condition including the time and temperature of acid hydrolysis was optimized to 105 ℃ for 150－180 min. Therefore, the method described in this study is highly efficient for the rapid degradation of curdlan into CRDO.