| 利用来源于Paenibacillus macerans的α-CGTase突变体Y89D生产α-环糊精 |
| |
| 引用本文： | 王宁,吴丹,陈晟,陈坚,吴敬. 利用来源于Paenibacillus macerans的α-CGTase突变体Y89D生产α-环糊精[J]. 食品科学, 2011, 32(3): 165-170. DOI: 10.7506/spkx1002-6630-201103039 |
| |
| 作者姓名： | 王宁 吴丹 陈晟 陈坚 吴敬 |
| |
| 作者单位： | 1.江南大学食品科学与技术国家重点实验室，江苏无锡 214122；2.江南大学生物工程学院，工业生物技术教育部重点实验室，江苏无锡 214122 |
| |
| 基金项目： | 国家杰出青年基金项目(20625619)；食品科学与技术国家重点实验室科研基金项目(SKLF-MB-200802)；国家“973”项目(2007CB 714036) |
| |
| 摘 要： | 对α- 环糊精葡萄糖基转移酶(α-CGTase)突变体Y89D 制备α- 环糊精的影响因素进行初步研究。其因素包括淀粉种类(马铃薯淀粉、玉米淀粉、木薯淀粉、可溶性淀粉)、加酶量、反应时间、pH 值、有机溶剂(乙醇、异丙醇、正丁醇、正癸醇)和温度。结果表明：选用5g/100mL 马铃薯淀粉、pH5.0、温度30℃、加酶量控制在 每克淀粉5U 左右，反应体系中加入体积分数5% 的正癸醇，反应6h 后，淀粉总转化率可达70%，其中α- 环糊精在产物中质量分数约为85%，转化产物中含有少量β- 环糊精(15%)，而极少生成γ- 环糊精。因此，α-CGTase突变体Y89D 在制备α- 环糊精工艺中具有很好的工业化应用前景。
|
| 关 键 词： | α-环糊精 α-环糊精葡萄糖基转移酶 有机溶剂 转化率 |
| 收稿时间： | 2010-04-30 |
Preparation of α-Cyclodextrin by Mutation Y89D of Cyclodextrin Glycosyltransferase from Paenibacillus macerans |
| |
| WANG Ning,WU Dan,CHEN Sheng,CHEN Jian,WU Jing. Preparation of α-Cyclodextrin by Mutation Y89D of Cyclodextrin Glycosyltransferase from Paenibacillus macerans[J]. Food Science, 2011, 32(3): 165-170. DOI: 10.7506/spkx1002-6630-201103039 |
| |
| Authors: | WANG Ning WU Dan CHEN Sheng CHEN Jian WU Jing |
| |
| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China ；2. Key Laboratory ofIndustrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
| |
| Abstract: | The factors for preparingα-cyclodextrin by mutation Y89D of α-cyclodextrin glycosyltransferase (α-CGTase) were investigated. The factors include starch types (potato, corn, cassava and soluble starch), enzyme amount, reaction time, pH, organic solvents (ethanol, isopropanol, n-butanol and n-decanol) and temperature. Results indicated that the optimal conditions were 5 g/100 mL potato starch, 5 U/g CGTase, pH 5.0, 30 ℃, 5% n-decanol and reaction time of 6 h. Under the optimal conditions, 70% conversion rate of starch to cyclodextrins was achieved, which included 85% α-cyclodextrin, 15% β-cyclodextrin and trace γ -cyclodextrin. Therefore, the mutation Y89D of α -CGTase has promising industrial application prospect. |
| |
| Keywords: | &alpha -cyclodextrin &alpha -cyclodextrin glycosyltransferase organic solvent conversion rate |
|
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
