| 桑叶多糖的分离纯化及对α - 葡萄糖苷酶的抑制活性 |
| |
| 引用本文： | 罗晶洁,王尉,曹学丽. 桑叶多糖的分离纯化及对α - 葡萄糖苷酶的抑制活性[J]. 食品科学, 2011, 32(3): 112-116. DOI: 10.7506/spkx1002-6630-201103027 |
| |
| 作者姓名： | 罗晶洁 王尉 曹学丽 |
| |
| 作者单位： | 北京工商大学化学与环境工程学院，北京市植物资源研究开发重点实验室，北京 100048 |
| |
| 摘 要： | 利用Sephadex-G50 从桑叶多糖中分离纯化得到两种不同多糖组分MLP1 和MLP2，经过高效凝胶色谱(GPC)分析表明，MLP1 的纯度为94.55%，重均相对分子质量MW 为11800，多分散性系数为1.25，MLP2 的纯度为96.64%，重均相对分子质量MW 为7630，多分散性系数为1.12。并利用α- 葡萄糖苷酶抑制活性体外筛选模型，对MLP1 和MLP2 进行活性研究，结果表明两个组分对α- 葡萄糖苷酶均具有较好的抑制活性。
|
| 关 键 词： | 桑叶 多糖 分离纯化 高效凝胶色谱 α- 葡萄糖苷酶 抑制活性 |
| 收稿时间： | 2010-06-03 |
Isolation,Purification and Anti-α-Glucosidase Activity of Polysaccharides from Mulberry Leaves |
| |
| LUO Jing-jie,WANG Wei,CAO Xue-li. Isolation,Purification and Anti-α-Glucosidase Activity of Polysaccharides from Mulberry Leaves[J]. Food Science, 2011, 32(3): 112-116. DOI: 10.7506/spkx1002-6630-201103027 |
| |
| Authors: | LUO Jing-jie WANG Wei CAO Xue-li |
| |
| Affiliation: | Beijing Key Laboratory of Plant Resources Research and Development, College of Chemical and Environmental Engineering,Beijing Technology and Business University, Beijing 100048, China |
| |
| Abstract: | Two kinds of polysaccharides (MLP1 and MLP2) were isolated and purified from mulberry leaves by Sephadex- G50. The gel permeation chromatographic (GPC) analysis revealed that the purity, relative molecular weight and polydispersity of MLP1 was 94.55%, 11800 and 1.25; Meanwhile, the purity, relative molecular weight and polydispersity of MLP2 was 96.64%, 7630 and 1.12, respectively. Moreover, obvious inhibitory effects of MLP1 and MLP2 on α-glucosidase were observed. |
| |
| Keywords: | mulberry leaves polysaccharide isolation and purification gel permeation chromatographic (GPC) &alpha -glucosidase inhibitory activity |
|
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
