Measurement of beta-amyloid peptides in specific cells using a photo thin-film transistor |
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Authors: | Chang-Beom Kim Cheol-Joo Chae Hye-Rim Shin Ki-Bong Song |
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Affiliation: | (1) IT Convergence Services Core Research Team, Electronics and Telecommunications Research Institute, 218 Gajeong-ro, Yuseong-gu, Daejeon, 305-700, South Korea |
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Abstract: | The existence of beta-amyloid Aβ] peptides in the brain has been regarded as the most archetypal biomarker of Alzheimer's
disease AD]. Recently, an early clinical diagnosis has been considered a great importance in identifying people who are at
high risk of AD. However, no microscale electronic sensing devices for the detection of Aβ peptides have been developed yet.
In this study, we propose an effective method to evaluate a small quantity of Aβ peptides labeled with fluorescein isothiocyanate
FITC] using a photosensitive field-effect transistor p-FET] with an on-chip single-layer optical filter. To accurately evaluate
the quantity of Aβ peptides within the cells cultured on the p-FET device, we measured the photocurrents which resulted from
the FITC-conjugated Aβ peptides expressed from the cells and measured the number of photons of the fluorochrome in the cells
using a photomultiplier tube. Thus, we evaluated the correlation between the generated photocurrents and the number of emitted
photons. We also evaluated the correlation between the number of emitted photons and the amount of FITC by measuring the FITC
volume using AFM. Finally, we estimated the quantity of Aβ peptides of the cells placed on the p-FET sensing area on the basis
of the binding ratio between FITC molecules and Aβ peptides. |
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Keywords: | Alzheimer''s disease beta-amyloid photosensitive field-effect transistor arsenic trisulfide optical filter |
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