| 优化TTC-脱氢酶还原法测定陶粒负载微生物活性 |
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| 引用本文： | 齐鲁青,汪晓军,詹德明.优化TTC-脱氢酶还原法测定陶粒负载微生物活性[J].现代化工,2012,32(3):93-96. |
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| 作者姓名： | 齐鲁青 汪晓军 詹德明 |
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| 作者单位： | 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广东广州51000 |
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| 基金项目： | 国家自然科学基金项目(51078149);广州市科技计划资助项目,(2009Z1-E751) |
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| 摘 要： | 利用氯化三苯基四氮唑(TTC)-脱氢酶还原法定量测定了曝气生物滤池中陶粒负载生物膜的活性,并对影响生物活性测定的因素进行了分析和优化。实验表明,经优化后得出最佳培养条件是:陶粒采用原位法制样,依次加入pH为8.0的Tris-HCl缓冲液,质量分数为0.4%的TTC溶液,0.1 mol/L葡萄糖溶液,在温度40℃下培养4 h,最后生成的三苯基甲臢(Triphenyl Formazone,TF)用甲苯萃取可以除去有色废水的颜色影响。采用TTC-脱氢酶还原法可以快速、方便的检测曝气生物滤池中陶粒负载生物膜的生物活性,并能很好的进行定量化测定。
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| 关 键 词： | TTC-脱氢酶还原法 曝气生物滤池 生物活性 优化 |
Study on determination of biofilm activity in BAF by TTC-dehydrogenase assay |
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| QI Lu-qing
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WANG Xiao-jun
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ZHAN De-ming.Study on determination of biofilm activity in BAF by TTC-dehydrogenase assay[J].Modern Chemical Industry,2012,32(3):93-96. |
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| Authors: | QI Lu-qing WANG Xiao-jun ZHAN De-ming |
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| Affiliation: | (School of Environmental Science and Engineering,South China University of Technology,Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters of Ministry of Education,Guangzhou 510006,China) |
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| Abstract: | The biofilms activity in Biological Aerated Filters(BAF) is quantitatively determined by 2,3,5-triphenyl tetrazolium chloride(TTC)-dehydrogenase assay and some major factors influencing the determination are studied in this paper.The optimum parameters are shown as follows:Tris-HCl buffers and 8.0 of pH,0.4%(mass fraction) of TTC and 0.1 mol/L of dextrose solution.The incubation time and temperature are 4 h and 40℃,respectively.The formazan reduction product can be efficiently extracted by methylbenzene,which can reduce the disturbance of dye color.This study demonstrates that the TTC-dehydrogenase assay can be successfully applied to the determination of biofilms activity in BAF. |
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| Keywords: | TTC-dehydrogenase assay biological aerated filters biofilm activity optimization |
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