| 毛细管电泳芯片分离DNA片段的实验研究 |
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| 引用本文： | 田丽,陈伟平,刘晓为,王喜莲. 毛细管电泳芯片分离DNA片段的实验研究[J]. 哈尔滨工业大学学报, 2004, 36(8): 1020-1022 |
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| 作者姓名： | 田丽 陈伟平 刘晓为 王喜莲 |
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| 作者单位： | 哈尔滨工业大学,MEMS中心,黑龙江,哈尔滨,150001;哈尔滨工业大学,MEMS中心,黑龙江,哈尔滨,150001;哈尔滨工业大学,MEMS中心,黑龙江,哈尔滨,150001;哈尔滨工业大学,MEMS中心,黑龙江,哈尔滨,150001 |
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| 基金项目： | 哈尔滨工业大学跨学科交叉性研究基金资助项目(HIT.MD2000 03). |
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| 摘 要： | 在研制出集成毛细管电泳芯片的基础上，采用自组建的毛细管电泳芯片-激光诱导荧光-光电倍增管(PMT)及4400数据采集处理系统，对IGF-1(500bp)MAF-box(1021bp)两种DNA样品片段进行分离测试实验，测试结果得出采用本测试体系所需要的最佳条件，为毛细管电泳芯片的实用化奠定了基础．
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| 关 键 词： | 毛细管电泳芯片 激光诱导荧光 DNA片段分离 |
| 文章编号： | 0367-6234(2004)08-1020-03 |
| 修稿时间： | 2003-01-27 |
Experiment research of DNA fragment separation using integrated capillary electrophoresis chip |
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| integrated capillary electrophoresis chip TIAN Li,CHEN Wei-ping,LIU Xiao-wei,WANG Xi-lian. Experiment research of DNA fragment separation using integrated capillary electrophoresis chip[J]. Journal of Harbin Institute of Technology, 2004, 36(8): 1020-1022 |
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| Authors: | integrated capillary electrophoresis chip TIAN Li CHEN Wei-ping LIU Xiao-wei WANG Xi-lian |
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| Abstract: | On the basis of designing and fabricating the integrated capillary electrophoresis chip, two pieces of DNA restriction fragments, IGF-1(500bp) and MAF-box(1021bp), are separated in a few seconds by using a self-setup detection system with capillary electrophoresis chips, laser-induced-fluorescence, the photo-multiplier tube and the 4400 data collection and processing system. Then the optimal tested condition is derivated by using this equipment. The results laid a strong foundation for putting the ICEC chip into practice. |
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| Keywords: | integrated capillary electrophoresis chip laser-induced-fluorescence DNA fragments separation |
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