|
|||||
|
|
| 柑橘皮渣降解菌的筛选及特性 | |
| 引用本文： | 李世忠,黄建国,李治玲,彭良志,李勇. 柑橘皮渣降解菌的筛选及特性[J]. 食品科学, 2014, 35(23): 188-192. DOI: 10.7506/spkx1002-6630-201423037 |
| 作者姓名： | 李世忠 黄建国 李治玲 彭良志 李勇 |
| 作者单位： | 1.西南大学资源环境学院，重庆 400715；2.中国农业科学院柑桔研究所，重庆 400715 |
| 基金项目： | 农业科技成果转化资金项目(2013GB2F100396);重庆市重大科技攻关计划项目(cstc2012gg-yyjs B80007);西南大学博士基金项目(SWU113018) |
| 摘 要： | 为有效处理柑橘皮渣，筛选高效降解柑橘皮渣的菌株。本实验从堆肥中分离得到5 株菌株，测定其香精油耐受性，选取两株耐受性较好的菌株测定纤维素酶和果胶酶活力，并进行柑橘皮渣降解实验。结果表明，菌株L207和L702在65 ℃条件下可正常生长，属于耐高温菌株；对柑橘香精油具有较好的耐受能力，其最大耐受体积分数为25.0%；2 株菌株的纤维素酶活力和果胶酶活力分别是对照菌株（Bacillus subtilis L520）的1.31、2.08 倍和12.68、7.30 倍。柑橘皮渣降解实验表明，菌株能快速高效分解纤维素和果胶。16S rDNA基因序列分析鉴定表明菌株L207和L702均属于地衣芽孢杆菌（Bacillus licheniformis）。 |
| 关 键 词： | 柑橘皮渣降解菌 柑橘精油 纤维素酶 果胶酶 16S rDNA |
Screening of Two Bacterial Strains Capable of Degrading Citrus Pomace and Their Characteristics |
|
| LI Shi-zhong,HUANG Jian-guo,LI Zhi-ling,PENG Liang-zhi,LI Yong. Screening of Two Bacterial Strains Capable of Degrading Citrus Pomace and Their Characteristics[J]. Food Science, 2014, 35(23): 188-192. DOI: 10.7506/spkx1002-6630-201423037 | |
| Authors: | LI Shi-zhong HUANG Jian-guo LI Zhi-ling PENG Liang-zhi LI Yong |
| Affiliation: | 1. College of Resources and Environment, Southwest University, Chongqing 400715, China;2. Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400715, China |
| Abstract: | This study was conducted to screen microorganisms degrading citrus pomace. Five strains of citrus pomacedegrading bacteria were screened from soil compost and tested for citrus essential oil toleranc. Two of these strains were found to have higher tolerance. Meanwhile, cellulase and pectinase activities of the two selected strains were also determined, and their ability to degrade citrus pomace was characterized. The experimental results showed that the selected strains L207 and L702 could grow normally at 65 ℃ as thermophilic stains. They could tolerate up to 25.0% aqueous ethanol and produce 1.31 and 2.08 times higher cellulase activity, and 12.68 and 7.30 times higher pectinase activity than the control Bacillus subtilis L520, respectively. Both strains were able to rapidly and efficiently degrade cellulose and pectin. 16S rDNA sequencing showed that they both belonged to Bacillus licheniformis. |
| Keywords: | citrus pomace degrading bacteria citrus essential oil cellulase pectinase 16S rDNA |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《食品科学》浏览原始摘要信息 | |
| 点击此处可从《食品科学》下载全文 | |