Different role of focA and focB encoding formate channels for hydrogen production by Escherichia coli during glucose or glycerol fermentation |
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| Affiliation: | 1. Department of Biophysics, Yerevan State University, 0025 Yerevan, Armenia;2. Department of Microbiology & Plants and Microbes Biotechnology, Yerevan State University, 0025 Yerevan, Armenia;1. State Key Laboratory of Hollow Fiber Membrane Materials and Processes (Tianjin Polytechnic University), Tianjin 300387, PR China;2. Institute of Medical Equipment, Academy of Military and Medical Sciences, Tianjin 300161, PR China;1. State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China;2. Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QR, United Kingdom;1. Advanced Energy Materials Processing Laboratory, Center for Energy Convergence Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea;2. Department of Material Science & Engineering, Korea University, Seoul 136-713, Republic of Korea |
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| Abstract: | The dependence of H2 production on the formate channels, FocA and FocB, by Escherichia coli at pH 5.5, 6.5 and 7.5 was shown using focA and focB mutants and comparing with the wild type. Moreover, effect of exogenous addition of formate (10 mM) on H2 production was allotted. The results acquired propose that during glucose fermentation formate import can occur through FocB at different pHs; external formate drives FocA to import direction. However, during glycerol fermentation formate might be imported through FocB, whereas formate is exported preferentially through FocA at pH 7.5. |
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| Keywords: | FocA and FocB formate channels Glucose or glycerol fermentation Biohydrogen production pH |
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