Molecular characterization of bacterial communities associated with sediments in the Laurentian Great Lakes |
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| Affiliation: | 1. Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI, USA;2. Center for Microbial Ecology and Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA;3. Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI, USA;1. Department of Biology, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada;2. Ontario Ministry of Natural Resources, Cooperative Freshwater Ecology Unit, Laurentian University, Sudbury, ON P3E 2C6, Canada;3. Department of Biology, Queen''s University, Kingston, ON K7L 3N6, Canada;1. Département de biologie et Québec -Ocean, Université Laval, 2056 Pavilion Alexandre-Vachon, Québec, QC G1K 7P4, Canada;2. Département de chimie-biologie, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, QC, G9A 5H7, Canada;1. The School of Environment and Natural Resources, The Ohio State University, Columbus, OH 43210-1085, USA;2. Statistical Consulting Service, The Ohio State University, Columbus, OH 43210-1085, USA |
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| Abstract: | The Laurentian Great Lakes freshwater ecosystem comprises a series of interconnected lakes exhibiting wide spatial differences in hydrogeochemistry, productivity, and allochthonous inputs of nutrients and xenobiotics. Bacterial communities associated with offshore sediments in this ecosystem have been rarely studied. We evaluated bacterial communities associated with sediments from 10 locations in four of the five Great Lakes (Lakes Superior, Michigan, Huron, and Ontario) by generating16S rRNA pyrosequence libraries. Pyrosequencing analysis revealed the presence of 26 bacterial phyla and proteobacterial classes among Great Lakes sediment samples. Actinobacteria, Acidobacteria, Betaproteobacteria, and Gammaproteobacteria were the most abundant groups of bacteria. Redundancy analysis was used to examine the role of sediment properties, including depth and chemical composition, in shaping bacterial community structure. One sample from Lake Huron was distinctly different from all other samples. Phylogenetic analysis revealed that the sample contained greater abundances of groups of bacteria associated with polluted environments. This study constitutes the most extensive examination of bacteria associated with Laurentian Great Lakes sediments and sheds useful insight into the microbial ecology of the Great Lakes. |
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