Co-fermentation of glucose,starch, and cellulose for mesophilic biohydrogen production |
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| Affiliation: | 1. Department of Chemical and Biochemical Engineering, Western University, London, ON N6A 5B9, Canada;2. Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 3V6, Canada;3. Department of Civil and Environmental Engineering, Western University, London, ON N6A 5B9, Canada;4. GreenField Specialty Alcohols, Chatham, ON N7M 5J4, Canada;5. Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada;1. Departamento de Física Aplicada, Cinvestav – Unidad Mérida, A.P. 73, Cordemex, 97310 Mérida, Yucatán, Mexico;2. Departamento de Recursos del Mar, Cinvestav – Unidad Mérida, A.P. 73, Cordemex, 97310 Mérida, Yucatán, Mexico;1. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China;2. School of Forestry, Northeast Forestry University, Harbin 150060, China;1. Laboratory of Advanced Catalysis and Environmental Technology, School of Science, Malaysia;2. Chemical Engineering Discipline, School of Engineering, Monash University, Jalan Lagoon Selatan, Bandar Sunway, 46150, Selangor Darul Ehsan, Malaysia;3. Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya, 50603 Kuala Lumpur, Malaysia;1. School of Energy Science and Engineering, Indian Institute of Technology Kharagpur, India;2. Dept. of Biotechnology, Indian Institute of Technology Kharagpur, India;1. Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, Biotechnology Department, Izmir, Turkey;2. Dokuz Eylul University, Department of Environmental Engineering, Izmir, Turkey |
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| Abstract: | The aim of this study was to assess the synergistic effects of co-fermentation of glucose, starch, and cellulose using anaerobic digester sludge (ADS) on the biohydrogen (H2) production and the associated microbial communities. Yields of H2 were 1.22, 1.00, and 0.15 mol H2/mol hexose-added in fermentation reactions containing glucose, starch, and cellulose as mono-substrates. The H2 yields were greater by an average of 27 ± 4% than expected in all the different co-substrate conditions, which affirmed that co-fermentation of different substrates improved the H2 potential. Glucose addition to starch and/or cellulose favored acetate synthesis, while cellulose degradation was associated with the propionate synthesis pathway. Illumina sequencing technology and bioinformatics analyses revealed operational taxonomic units (OTUs) in the Phyla Bacteroides, Chloroflexi, Firmicutes, Proteobacteria, Spirochaetes, Synergistes, and Thermotogae were common in mono- and co-substrate batches. However, OTUs in the Phyla Acidobacteria, Actinobacteria, and Bacteroidetesee were unique to only the co-substrate conditions. |
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| Keywords: | Co-fermentation Biohydrogen Glucose Starch Cellulose Microbial community structure |
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