Phytosterols in banana (Musa spp.) flower inhibit α‐glucosidase and α‐amylase hydrolysations and glycation reaction |
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| Authors: | Zhanwu Sheng Haofu Dai Siyi Pan Binling Ai Lili Zheng Xiaoyan Zheng Witoon Prinyawiwatkul Zhimin Xu |
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| Affiliation: | 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China;2. Haikou Key Laboratory of Banana Biology, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China;3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China;4. School of Nutrition and Food Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA, USA |
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| Abstract: | Three phytosterols were isolated from Musa spp. flowers for evaluating their capabilities in inhibiting glucosidase and amylase activities and glycation of protein and sugar. The three phytosterols were identified as β‐sitosterol (PS1), 31‐norcyclolaudenone (PS2) and (24R)‐4α, 14α, 4‐trimethyl‐5α‐cholesta‐8, 25(27)‐dien‐3β‐ol (PS3). IC50 values (the concentration of inhibiting 50% of enzyme activity) of PS1, PS2 and PS3 against α‐glucosidase were 283.67, 11.33 and 43.10 μg mL?1, respectively. For inhibition of α‐amylase, the IC50 values of PS1, PS2 and PS3 were 52.55, 76.25 and 532.02 μg mL?1, respectively. PS1 was an uncompetitive inhibitor against α‐amylase with Km at 5.51 μg mL?1, while PS2 and PS3 exhibited a mixed‐type inhibition with Km at 52.36 and 2.49 μg mL?1, respectively. PS1 and PS2 also significantly inhibited the formation of advanced glycation end products (AGEs) in a BSA–fructose model. The results suggest that banana flower could possess the capability in prevention of the diseases associated with abnormal blood sugar and AGEs levels, such as diabetes. |
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| Keywords: | Banana flower glycation Maillard reaction
Musa spp
phytosterol α ‐amylase α ‐glucosidase |
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