Physical,antimicrobial and antibiofilm properties of Zataria multiflora Boiss essential oil nanoemulsion |
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Authors: | Nasim Shahabi Hossein Tajik Mehran Moradi Mehrdad Forough Parya Ezati |
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Affiliation: | 1. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azarbaijan 1177, Iran;2. Department of Analytical Chemistry, Faculty of Chemistry, Urmia University, Urmia, West Azarbaijan 1177, Iran |
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Abstract: | It was evaluated physical, antibacterial and antibiofilm properties of Zataria multiflora Boiss essential oil (ZEO) and its nanoemulsion. Long‐term stability of nanoemulsion prepared by emulsion phase inversion was satisfying based on low narrow size distribution (polydispersity index ?0.2) and low droplet size (200 nm) over 21 days of storage. Nanoemulsion showed lower minimum inhibitory concentration (MIC) on Listeria monocytogenes (2500 µg mL?1) than Salmonella Typhimurium (5000 µg mL?1). Killing kinetics study revealed that nanoemulsion was more effective in inhibiting the growth of bacteria in milk than culture media. Both bacteriostatic and bactericidal effects were observed depending on the type of bacteria, nanoemulsion concentration and the time of exposure. Nanoemulsion at 4×MIC concentration reduced 64% and 75% of one‐day‐old biofilm of L. monocytogenes and S. Typhimurium, respectively. In conclusion, nanoemulsion revealed antimicrobial activity, but converting the ZEO to nanoemulsion did not improve its antibacterial activity; however, antibiofilm properties were enhanced. |
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Keywords: | Antibacterial antibiofilm nanoemulsion stability Zataria multiflora essential oil |
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