脂肪酶催化动力学拆分大分子手性化合物的研究进展

脂肪酶已广泛用于制备手性化合物的动力学拆分中，但大多数酶催化拆分外消旋化合物，特别是大分子手性化合物的对映选择性不很理想。目前，提高脂肪酶对映选择性的研究主要在改造脂肪酶蛋白结构、优化体系的反应条件、改善反应过程以及对映选择性抑制等方面进行。本文主要介绍了几种改善脂肪酶催化动力学拆分大分子手性化合物对映选择性的合理方法。

Research Progress of Lipase-Catalyzed Kinetic Resolutions of very Bulky Chiral Compounds

Lipase has been extensively used in kinetic resolution for producing optical pure compounds. but the enantio selectivity of a majority of lipase-catalyzed resolutions of racemize chiral compounds is not so ideal . At present, the research work of upgrading enantio selectivity of lipase is focused on reformation of lipase structure , reaction conditions optimization, improvement of asymmetic reaction process and enantio selective inhibitation. Some traditional and new rational approaches such as substrate design and limitation of size of substrate, high temperature biocatalysis and low temperature biocatalysis are introduced.