| HPLC-UV-FTICRMS/MS~n法分离鉴定葛根素原料药中4种有关物质 |
| |
| 引用本文： | 王鹏远,吴彩胜,吴松,张金兰.HPLC-UV-FTICRMS/MS~n法分离鉴定葛根素原料药中4种有关物质[J].质谱学报,2010,31(3):143-151. |
| |
| 作者姓名： | 王鹏远 吴彩胜 吴松 张金兰 |
| |
| 作者单位： | 中国医学科学院,北京协和医学院,药物研究所，北京100050 |
| |
| 基金项目： | 卫生行业科研专项项目 |
| |
| 摘 要： | 采用高效液相色谱-紫外检测器-傅里叶变换离子回旋共振质谱(HPLC-UV-FTICRMS/MSn)技术获得了葛根素及其有关物质的MSn和HRMS数据,分析其可能的裂解机制,推断有关物质的结构,并依据HRMS数据确证了有关物质及其部分碎片的分子组成,最终鉴定葛根素原料药中4种有关物质,其准分子离子峰M+H]+分别为m/z433.112 95、433.112 92、417.118 01和417.118 01,通过质谱裂解机制研究以及与文献数据的对比,推测它们的结构分别为3’-羟基葛根素、染料木素-8-C-葡萄糖苷、新葛根素B和新葛根素A。
|
| 关 键 词： | 葛根素 电喷雾电离（ESI） 傅里叶变换离子回旋共振质谱(FTICR-MS) 结构鉴定 |
Separation and Characterization of 4 Related Impurities in Puerarin Bulk Drug by HPLC-UV-FTICRMS/MSn |
| |
| WANG Peng-yuan,WU Cai-sheng,WU Song,ZHANG Jin-lan.Separation and Characterization of 4 Related Impurities in Puerarin Bulk Drug by HPLC-UV-FTICRMS/MSn[J].Journal of Chinese Mass Spectrometry Society,2010,31(3):143-151. |
| |
| Authors: | WANG Peng-yuan WU Cai-sheng WU Song ZHANG Jin-lan |
| |
| Affiliation: | Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China |
| |
| Abstract: | The molecular structures and mechanism for the fragmentations of the related impurities were proposed by the MSn and HRMS results obtained by high performance liquid chromatography-ultraviolet detector-fourier transform ion cyclotron resonace mass spectrometry(HPLC-UV-FTICRMS/MSn).The molecular compositions of the related impurities and some of the fragmentations were confirmed by the HRMS datas.Four related impurities in puerarin bulk drug were separated.Their + ion peaks are m/z 433.112 95,433.112 92,417.... |
| |
| Keywords: | puerarin electrospray ionization(ESI) fourier transform ion cyclotron resonance mass spectrometry(FTICR-MS) structure characterization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《质谱学报》浏览原始摘要信息 |
|
点击此处可从《质谱学报》下载全文 |
