麦胚蛋白酶解产物对过氧化氢诱导PC12细胞损伤的抗氧化作用

Wheat germ protein isolate hydrolysates (WGPIH) were prepared under different enzymolysis time and the antioxidant effects of different WGPIH against the H₂O₂-induced oxidative stress in PC12 cells were compared through MTT method combined with their antioxidant activities in vitro, and the changes of the PC12 cells surface microstructures were observed by SEM. The results showed that the hydrolysate WGPIH5 produced by hydrolyze for 240 min possessed the highest antioxidant activity, its IC₅₀value for ferrous iron-chelating activity was（0.37±0.05）mg/mL；TEAC value （Trolox） reached（0.81±0.01）mmol/L with 5 mg/mL WGPIH5; the autooxidation of linoleic acid was significantly inhibited by WGPIH5；WGPIH5 was able to attenuate H₂O₂

Antioxidant effects of wheat germ protein isolate hydrolysates against H2O2-induced oxidative stress in PC12 cells