| HPLC-ESLD法分离测定大豆磷脂酰胆碱 |
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| 引用本文： | 曹栋. HPLC-ESLD法分离测定大豆磷脂酰胆碱[J]. 中国油脂, 2005, 30(12): 43-45 |
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| 作者姓名： | 曹栋 |
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| 作者单位： | 江南大学食品学院,214036,江苏省无锡市惠河路170号 |
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| 摘 要： | 通过HPLC-ELSD对大豆磷脂中的大豆磷脂酰胆碱进行了分离和检测.使用硅胶柱,以三氯甲烷-甲醇(88/12,V/V)为流动相,大豆磷脂酰胆碱在10 min内出峰.该法具有比HPLC-UV有更少的干扰、更高的检测灵敏度和更节省时间,同时该法具有良好的线性关系、分析准确度和分析精密度.该法可以方便地检测大豆磷脂中大豆磷脂酰胆碱的含量.
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| 关 键 词： | 大豆磷脂 磷脂酰胆碱 |
| 文章编号： | 1003-7969(2005)12-0043-03 |
| 收稿时间： | 2005-05-30 |
| 修稿时间： | 2005-05-30 |
Separation and determination of soybean phosphatidylcholine in soybean phospholipid by HPLC- ESLD |
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| CAO Dong. Separation and determination of soybean phosphatidylcholine in soybean phospholipid by HPLC- ESLD[J]. China Oils and Fats, 2005, 30(12): 43-45 |
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| Authors: | CAO Dong |
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| Affiliation: | School of Food, Jiangnan University, 214036 Jiangsu Wuxi, China |
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| Abstract: | Soybean phosphatidylcholine in soybean phospholipid was separated and the content of soybean phosphatidylcholine in soybean phospholipid was determined by HPLC - ESLD. The silicon column was used and CHCl3/ MeOH(88/12) was used as flowing phase. On this condition the position of soybean phosphatidylcholine is inside 10 min. The method spent shorter time than that of HPLC - UV. At the same time the sensitivity, linearity, veracity of the method were very good. The content of soybean phosphatidylcholine in soybean phospholipid can be easy determined by the method. |
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| Keywords: | HPLC-ESLD |
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