Rhodamine-based fluorescent sensor for mercury in buffer solution and living cells |
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Authors: | Huan-Huan Wang Lin XueCai-Lan Yu Yuan-Yu Qian Hua Jiang |
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Affiliation: | a Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China b Emergency Department of Chinese PLA General Hospital, Beijing 100853, PR China c Graduate School of Chinese Academy of Sciences, Beijing, PR China |
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Abstract: | A novel fluorescent sensor based on thiooxorhodamine B has been prepared to detect Hg2+ in aqueous buffer solution. It demonstrates high selectivity for sensing Hg2+ with about 383-fold enhancement in fluorescence emission intensity and micromolar sensitivity (Kd = 7.5 × 10−6 mol L−1) in comparison with alkali and alkaline earth metal ions (K+, Na+, Mg2+, Ca2+) and other transition metal ions (Mn2+, Ni2+, Co2+, Cu2+, Zn2+, Cd2+, Ag+, Pb2+, Cr3+, Fe3+). Meanwhile the distinct color changes and rapid switch-on fluorescence also provide ‘naked eyes’ detection for Hg2+ over a broad pH range. Moreover, such sensor is cell-permeable and can visualize the changes of intracellular mercury ions in living cells using fluorescence microscopy. |
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Keywords: | Fluorescent sensor Selectivity Mercury Rhodamine Cellular imaging pH stable |
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