胎肝-骨髓程序移植提高移植效果

为进一步提高骨髓移植效果。以昆明种小鼠急性放射病为模型,进行了胎肝－骨髓程序移植（ＦＬ－ＢＭＳＴ）后小鼠活存及造血重建、急性移植物抗宿主病（ＧＶＨＤ）的研究。胎肝－骨髓程序移植 １ｘ １０６个骨髓细胞时,于照射后第 １７ｄ外周血白细胞、骨髓有核细胞计数接近正常,ＣＦＵ－Ｅ、 ＣＦＵ－ＧＭ、 ＣＦＵ－Ｆ、 ＣＦＵ－Ｓ已达正常; ＧＶＨＤ较一次骨髓移植组轻;活存率达６０％,显著高于一次骨髓移植组。胎肝-骨髓程序移植既充分利用了每次腾出的“龛位”（Ｎｉｃｈｅ）,又通过移植胎肝基质细胞改善了“龛位”,增加了“龛位”,从而增加了干、祖细胞的植入并加快增殖分化,减少了移植细胞数,提高了移植效果;是一种较好的移植新方法。

ENHANCEMENT OF THE GRAFTING EFFICIENCY BY THE NEW METHOD OF FETAL LIVER-BONE MARROWSCHEDULED TRANSPLANTATION

To enhance the grafting efficiency of bone marrow transplantation. Lethally Irradiated recipient Kunming mice were transplantation with fetal liver-bone marrow scheduled transplantation. (FL-BMST) The numbers of WBC, nucleated cells were near to normal Ievel 17d after irradiation in FL-BMST group transplantation with 1 x 10~6 bone marrow cells. the indexes of CFU-E, CFU - GM, CFU-F, CFU-S, were returned to normal; the degree of GVHD in the FL-BMST group was slighter than that in sing bone marrow transplantation group: and the survival rate of mice was 60%, which was significantly higher than that of routine single bone marrow transplantation group. "Niches" vacated each time could be fully used and be improved, be increased by fetal liver-bone marrow scheduled transplantation, so the homing of stem cells was increased, and the number of transplanted bone marrow cells could be decreased. So this new method was a better method than routine bone singe marrow transplantation.