| 黑曲霉液体发酵产木聚糖酶的研究 |
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| 引用本文： | 吕世锋,任建伟,张满,杨付伟,阎振丽.黑曲霉液体发酵产木聚糖酶的研究[J].山东化工,2011,40(9):8-12,19. |
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| 作者姓名： | 吕世锋 任建伟 张满 杨付伟 阎振丽 |
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| 作者单位： | 河南天冠企业集团有限公司,河南南阳,473000 |
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| 基金项目： | 国家科技支撑计划项目(2007BAD66B01) |
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| 摘 要： | 通过单因素实验及响应面方法对黑曲霉菌株产木聚糖酶发酵条件进行了研究,结果表明:当培养基为麸皮40g/L,(NH4)2 SO4 4g/L,KH2PO4 3g/L,MgSO4 0.8g/L,吐温80 1g/L;初始pH值5.0,接种量8.0%,培养温度30℃;此条件下发酵木聚糖酶酶活达1456.27IU/mL。
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| 关 键 词： | 黑曲霉 木聚糖酶 酶活力 培养基 发酵条件 响应面 |
Study on Aspergillus Niger Producing Xylanase in Submerged Fermentation |
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| LV Shi-feng,REN Jian-wei,ZHANG Man,YANG Fu-wei,YAN Zhen-li.Study on Aspergillus Niger Producing Xylanase in Submerged Fermentation[J].Shandong Chemical Industry,2011,40(9):8-12,19. |
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| Authors: | LV Shi-feng REN Jian-wei ZHANG Man YANG Fu-wei YAN Zhen-li |
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| Affiliation: | LV Shi-feng,REN Jian-wei,ZHANG Man,YANG Fu-wei,YAN Zhen-li (Henan Tianguan Group Co.,Ltd.,Nanyang 473000,China) |
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| Abstract: | The fermentation conditions of aspergillus niger producing xylanase were studied by single facter experiment and response surface method. The results were as follows ： bran 40 g / L , （ NH4 ） 2 SO4 4g/L, KH2PO4 4g/L, MgSO4 0.8g/L, Tween 80 1 g/L, initial pH value 5.0, inoculation volume 8.0%, temperature 30℃, under these conditions, the activity of xylanase was reached 1456.27IU/mL. |
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| Keywords: | aspergillus niger xylanase enzyme activity culture medium fermentation condition response surface |
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