Wheat bran globulins: Competitive inhibitors of mushroom tyrosinase |
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| Authors: | Angel Martín Ortíz-Estrada Jorge Nemesio Mercado-Ruiz Jesús Manuel García-Robles Alma Rosa Islas-Rubio Ana María Mendoza-Wilson René Renato Balandrán-Quintana |
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| Affiliation: | 2. Instituto Tecnológico de Acapulco. Av. Instituto Tecnológico s/n, Crucero de Cayaco. Acapulco, Gro., México
1. Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal. Carretera a Ejido La Victoria Km. 0.6., PO Box 1735, Hermosillo, Sonora, 83304, México
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| Abstract: | The inhibitory capacity of the globulin fraction of wheat bran against the diphenolase activity of mushroom tyrosinase, using l-tyrosine as substrate, was evaluated. Enzyme kinetics was monitored in the presence of globulin solutions by measuring the absorbance at 475nm. Lineweaver-Burk plots were drawn in order to determine Vmax, Km, and type of inhibition. Results showed that globulins from wheat bran competitively inhibited, the activity of mushroom tyrosinase with a KI of 0.79%(w/v). The degree of inhibition was 24% at 2 mM of the substrate L-tyrosine. |
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