| 响应曲面法优化刺山柑水溶性多糖提取工艺及其抗氧化性能研究 |
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| 引用本文： | 陈燕勤,陈德军,张玲,李祥. 响应曲面法优化刺山柑水溶性多糖提取工艺及其抗氧化性能研究[J]. 化学与生物工程, 2013, 0(10): 24-29,53 |
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| 作者姓名： | 陈燕勤 陈德军 张玲 李祥 |
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| 作者单位： | [1]新疆农业大学化学工程学院,新疆乌鲁木齐830052 [2]新疆大学化学化工学院,新疆乌鲁木齐830046 |
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| 基金项目： | 国家自然科学基金资助项目（21066013） |
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| 摘 要： | 以刺山柑果实为原料,在单因素实验的基础上,选择提取温度、提取时间和液料比为影响因子,以刺山柑水溶性多糖提取率为响应值,应用Box-Behnken中心组合方法建立数学模型,利用响应曲面法确定超声辅助提取刺山柑水溶性多糖的优化工艺条件为：提取温度85℃、提取时间89 min、液料比30∶ 1（mL∶g）,在此条件下刺山柑水溶性多糖平均提取率为8.89％.提取得到的刺山柑水溶性多糖对羟基自由基（·OH）和超氧阴离子自由基（·O2-）均有一定的清除作用,具有一定的抗氧化活性.
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| 关 键 词： | 刺山柑 超声辅助提取 水溶性多糖 响应曲面法 抗氧化性能 |
Study on Optimization of Extraction of Capparis Spinosa L.Water-Soluble Polysaccharides by Response Surface Methodology and Its Antioxidant Activity |
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| CHEN Yan-qin,CHEN De--jun,ZHANG Ling,LI Xiang. Study on Optimization of Extraction of Capparis Spinosa L.Water-Soluble Polysaccharides by Response Surface Methodology and Its Antioxidant Activity[J]. Chemistry & Bioengineering, 2013, 0(10): 24-29,53 |
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| Authors: | CHEN Yan-qin CHEN De--jun ZHANG Ling LI Xiang |
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| Affiliation: | 1. College of Chemical Engineering, Xinj iang Agricultural University, Urumqi 830052, China 2. College of Chemistry and Chemical Engineering, Xinjiang University ,Urumqi 830046,China) |
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| Abstract: | Response surface methodology was used for optimizing the ultrasound-assisted extraction tech- nology of water-soluble polysaccharides from fruit of Capparis spinosa L.. Based on single factor experiment, extraction temperature, extraction time and liquid-solid ratio were selected as influencing factors. The experi- ment mathematical model was arranged according to Box-Behnken central composite design. The optimum ex- traction conditions were as follows, extraction temperature of 85℃, extraction time of 89 rain,liquid-solid ratio of 30 ： l（mL· g）. Under above conditions,the extraction rate of water-soluble polysaccharides was 8.89M. It was found that hydroxyl free radical （ · OH） and superoxide anion free radical （ · O2- ） were cleaned obviously by Capparis spinosa L. water-soluble polysaccharides,which had significant antioxidant activity. |
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| Keywords: | Capparis spinosa L. ultrasound-assisted extraction, water-soluble polysaccharides responsesurface methodology~ antioxidant activity |
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