Significant population genetic structuring in Rhyzopertha dominica across Turkey: Biogeographic and practical implications |
| |
| Affiliation: | 1. School of Biological Sciences, The University of Queensland, Brisbane, Queensland, 4072, Australia;2. Department of Zoology, University of Otago, P.O. Box 56, Dunedin, 9054, New Zealand;3. Department of Agricultural Biotechnology, Faculty of Agriculture, ISUBU, Isparta, 32260, Turkey;4. Department of Agriculture and Fisheries, EcoSciences Precinct, GPO Box 267, Brisbane, Queensland, 4001, Australia;1. Balochistan Agricultural Research Centre, Brewery Road, Western Bypass, Quetta, Pakistan;2. Institute of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North, New Zealand;3. Pakistan Agricultural Research Council, Plot No. 20, G-5/1, Islamabad, Pakistan;1. Instituto Biológico, Unidade Laboratorial de Referência Em Pragas Urbanas, Av. Conselheiro Rodrigues Alves, 1252, São Paulo, SP, CEP 04014-002, Brazil;2. Instituto Biológico, Laboratório de Acarologia, Alameda Dos Vidoeiros, 1097, Campinas, SP, CEP 13101-680, Brazil;1. Ahi Evran University, Faculty of Agriculture, Bağbaşı, 40100, Kırşehir, Turkey;2. Ahi Evran University, Department of Environmental Engineering, Bağbaşı, 40100, Kırşehir, Turkey;1. Morden Research and Developmental Centre, Agriculture and Agri-Food Canada, c/o Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada;2. Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada;1. Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, 768019, Odisha, India;2. Plant Molecular Breeding and Functional Genomics Laboratory, Department of Biotechnology, Central University of Rajasthan, NH-8, Bandarsindri, 305817, Kishangarh, Ajmer, Rajasthan, India;3. Department of Bioscience and Bioinformatics, Khallikote University, Berhampur, 760001, Odisha, India |
| |
| Abstract: | Rhyzopertha dominica is a key pest of stored grain. Understanding the movement of this beetle on broad geographic scales is crucial, particularly when developing strategies to prevent the spread of phosphine resistance. We assessed population genetic structuring in this pest across Turkey, using a combination of mitochondrial (cytochrome oxidase I) and microsatellite markers. In addition, we screened samples for Wolbachia, as this endosymbiont has previously been suggested to be associated with low mitochondrial genetic diversity in this beetle. Mitochondrial genetic diversity was low, with only six haplotypes identified. The genetic diversity was, however, substantially higher than that previously found in Australia or India, suggesting that R. dominica may have originated in the Middle East. Wolbachia were detected only at a single site, indicating they are not impacting the mitochondrial genetic diversity of R. dominica across Turkey. Microsatellite markers indicated there is significant geographic genetic structuring across Turkey, even among sites less than 100 km apart, suggesting there is little movement of beetles across regions within the country. This contrasts with the significantly higher levels of gene-flow found in Australia and the United States. We suggest that the limited movement of beetles across Turkey may be due to a combination of the historically localised agricultural practices (which limits anthropogenic movement among regions), and the mountainous landscape (which limits active flight among regions). Our results demonstrate that the movement of stored product pests may differ significantly across studies conducted in different countries. As a consequence, phosphine resistance management strategies must incorporate region specific information on the extent of beetle movement. |
| |
| Keywords: | Cytochrome oxidase I Genetic diversity Microsatellites Phosphine resistance |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
