Identification,biochemical characterization and biological role of defense proteins from common bean genotypes seeds in response to Callosobruchus maculatus infestation |
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| Affiliation: | 1. Universidade Estadual do Norte Fluminense Darcy Ribeiro, Laboratório de Melhoramento Genético Vegetal, Centro de Ciências e Tecnologias Agropecuárias, Brazil;2. Universidade Estadual do Norte Fluminense Darcy Ribeiro, Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Brazil;3. Universidade Estadual do Norte Fluminense Darcy Ribeiro, Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Brazil;1. Department of Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P, 250110, India;2. Department of Soil Science, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, U.P, 250110, India;1. Universidade Estadual Paulista “Júlio de Mesquita Filho”, Departmento de Fitossanidade, Via de Acesso Professor Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, Brazil;2. Instituto Agronômico de Campinas, Avenida Barão de Itapura, 1481, 13020-902, Brazil;3. Universidade Federal de São Carlos, Departmento de Química, Rod. Washington Luiz, Km 235, Pocket Box 676, São Carlos, SP 13.565-905, Brazil;1. Rani Lakshmi Bai Central Agricultural University, Jhansi, India;2. ICAR- Indian Institute of Pulses Research, Kanpur, India;1. School of Pharmacy, University of Lincoln, Lincoln, LN6 7TS, UK;2. School of Life Sciences, University of Lincoln, Lincoln, LN6 7TS, UK;3. Applied Materials Technology Ltd, Lyndon Business Park, Farrier Road, Lincoln, LN6 3RU, UK;1. Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Campus 6th Floor, Burma Colony, Karaikudi, 630 004, Tamil Nadu, India;2. Department of Biotechnology, Dr.Umayal Ramanathan College for Women, Karaikudi, 630 003, Tamil Nadu, India |
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| Abstract: | Common bean is a legume of significant socioeconomic importance and is cultivated worldwide. This crop is affected by several pests and diseases, which cause considerable economic losses and reduce yield. In recent years, several studies have demonstrated the role of proteins and peptides with activity against a wide range of insects and pathogens. The objective of this work was to identify defense proteins, such as antimicrobial peptides, protease and amylases inhibitors in common bean genotypes and evaluate the relationship of these proteins with Phaseolus vulgaris seed resistance to Callosobruchus maculatus infestation. Nineteen common bean genotypes were subjected to protein extraction, pH 5.4, and precipitation with ammonium sulfate at 70% saturation. The obtained extracts were separated by tricine gel electrophoresis. Experiments were carried out with natural seeds of common beans and artificial seeds (Vigna unguiculata seeds covered with seed coats of common beans) to evaluate the rate of oviposition and development of the insect species Callosobruchus maculatus. Lipid-transfer proteins were identified in nine genotypes whereas defensins were present in all genotypes. The inhibitory activity of α-amylases and trypsin and fungal development were determined in crude extracts (50 μg mL?1). The results also indicated that the extracts from all bean genotypes inhibited the activity of human salivary α-amylase and C. maculatus larval α-amylase. Except for the extracts of four genotypes, all other extracts inhibited trypsin activity. None of the extracts from the evaluated bean genotypes inhibited the growth of tested fungi. Natural seeds from all genotypes did not inhibit insect oviposition, however, the larvae did not survive after feeding on these seeds. Artificial seeds containing seed coat flour of all genotypes inhibited the oviposition of C. maculatus, indicating that the seed coat was also repellent to insect. |
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| Keywords: | Plant defensin Defense proteins Seed coat Proteinase inhibitor |
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