Rice bean (Vigna umbellata Thunb. Ohwi and Ohashi) protection against Callosobruchus maculatus F.” by presence of protein profile |
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| Affiliation: | 1. Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India;2. Department of Entomology, College of Agriculture, Punjab Agricultural University, Ludhiana, 141004, Punjab, India;3. Department of Biochemistry, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana, 141004, Punjab, India;1. Laboratory of Agricultural Zoology and Entomology, Department of Crop Science, Agricultural University of Athens, 75 Iera Odos str., 11855, Athens, Attica, Greece;2. Laboratory of Organic Chemistry, Department of Chemistry, University of Ioannina, Panepistimioupolis, 45110, Ioannina, Greece;3. Laboratory of Biological Control of Pesticides, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 8 Stefanou Delta str., 14561, Kifissia, Attica, Greece;4. Laboratory of Agricultural Entomology, Department of Entomology and Agricultural Zoology, Benaki Phytopathological Institute, 8 Stefanou Delta str., 14561, Kifissia, Attica, Greece;5. School of Pharmacy, University of Camerino, Via Sant’Agostino 1, 62032, Camerino, Italy;6. Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124, Pisa, Italy;1. Department of Entomology, Kansas State University, Manhattan, KS, 66506, USA;2. Department of Pesticide Chemistry and Technology, Faculty of Agriculture, 21545-El-Shatby, Alexandria University, Alexandria, Egypt;3. Altria Client Services LLC, Research, Development & Engineering, 601 E Jackson Street, Richmond, VA, USA;1. USDA, Agricultural Research Service, Center for Grain and Animal Health Research, 1515 College Ave, Manhattan, KS, 66502, USA;2. Laser for Innovative Solutions (L4iS), Suite 261, 200 Innovation Boulevard, State College, PA, 16803, USA;3. Department of Engineering, University of Southern Maine, 37 College Ave, Gorham, ME, 04038, USA;1. Department of Biosystems Machinery Engineering, Chungnam National University, Daejeon, 34134, South Korea;2. Department of Applied Biology, Chungnam National University, Daejeon, 34134, South Korea |
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| Abstract: | To identify defence responses of rice bean against Callosobruchus maculatus, 10 genotypes of rice bean and a mungbean variety, PAU 911 as susceptible check were evaluated. The rice bean genotypes were found to restrain the growth and development of C. maculatus at different larval stages. To justify these results, seeds of different test genotypes were examined for the presence of plant protease inhibitors which inhibit insect proteases causing reduction in the availability of amino acids necessary for their growth and development. Trypsin, chymotrypsin and cysteine protease inhibitor content was significantly higher in rice bean genotypes which ranged from 299.99 to 342.97 TIU g?1 seeds, 235.07 to 251.45 CIU/g seeds and 1060.70 to 1574.99 CPIU/g seeds, respectively as compared to mungbean variety, PAU 911 (235.52 TIU/g seeds, 217.91 CIU/g seeds and 499.99 CPIU/g seeds, respectively). The protein profile showed a polypeptide band of 25–30 kDa corresponding to presence of protease inhibitors in rice bean genotypes except PAU 911. The results indicate that protease inhibitors in different genotypes of rice bean are very effective against C. maculatus. |
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| Keywords: | Bruchid Protease inhibitors Resistant |
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