Enhancing spray-drying tolerance of Lactobacillus bulgaricus via non-sporeforming dormancy induction |
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| Affiliation: | 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China;2. Center for Food and Nutrition Research, Hisense home appliances group Co., Ltd, Qingdao 266000, China;1. Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, PR China;2. Key Laboratory of Dairy Products Processing, Ministry of Agriculture, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, PR China;1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;2. Hebei Meiyesiwei Biotechnology Co. Ltd., 072650, China;1. Department of Animal Health and Public Health, Veterinary Medicine Faculty, Universidad Nacional Mayor de San Marcos, San Borja, 15021 Lima, Peru;2. Department of Food Technology, Universitat Politècnica de València, Cami de Vera, s/n, E46022, Valencia, Spain;3. Food Technology Dept. Section, Veterinary Faculty, Complutense University, 28040 Madrid, Spain;1. College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China;2. Department of Biological Systems Engineering, Washington State University, 213 L.J. Smith Hall, Pullman, WA 99164-6120, USA;1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China |
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| Abstract: | Employing 0.64 mg/mL of rifampin (RFP) for dormancy induction of Lactobacillus bulgaricus sp 1.1, a 2.5-fold survival rate was achieved after spray drying, while the dried bacteria displayed delayed acidifications. Under the same condition for dormancy treatment, division analysis indicated that high proportions of cells would enter into a dormant state with around 90% of non-proliferating bacteria in the original population. Cells shrank with median diameters decreasing from 3.23 to 2.98 μm. The Raman spectrum revealed a lower ATP level, varied protein expression profiles and conformations and saccharides accumulation in bacteria. Protein aggregates formed in dormancy-induced cells may contribute to increased heat resistance which showed a 2 °C rise in the denaturing temperature in the 0.64 mg/mL group. Therefore, dormancy treatments may be developed as a protective strategy for spray-dried L. bulgaricus.Industrial relevanceEnhancing the drying tolerance of bacteria is one of the crucial strategies to adapt the industrial production of spray-dried LAB. Our study proved that the non-sporeforming dormancy pre-treatment can promote the spray-dried viability of L. bulgaricus by triggering suppressed metabolism activity and protein aggregation. The non-sporeforming dormant state has potential to be exploited in industrial production of spray-dried LAB with a high viable cell density to reduce the cost of probiotics and related products. |
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