Regression analysis of amino acid substitutions and factor IX activity in hemophilia B

Hemophilia B is caused by a deficit or decreased activity of factor IX. Mutation in factor IX is made up of a majority of amino acid substitutions. We examined the relation between the activation level of factor IX and the category of amino acid substitution with regression analysis. As parameters, we used four physical-chemical parameters of amino acids and Dayhoff’s PAM matrix. In addition, the average of the activation level with the same amino acid substitution was used for the analysis. We analyzed the relationship between variations containing cysteine or factor IX’s seven regions and activity levels. This work was presented in part at the 13th International Symposium on Artificial Life and Robotics, Oita, Japan, January 31–February 2, 2008