The sulfide electrode in bacterial studies |
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Authors: | Talya Bergstein-Ben Dan Tonnies Frevert Benzion Cavari |
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Affiliation: | 1. Kinneret Limnological Laboratory, P.O. Box 345 Tiberias 14-102, Israel;2. Chair of Hydrology, University of Bayreuth, P.O. Box 3008, 8580 Bayreuth, West Germany |
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Abstract: | The applicability of a glass/Ag, Ag2S (silver sulfide coated silver) electrode, which is capable of selectively detecting the H2S fraction of total reduced inorganic sulfur (Stot) concentration in biological systems is demonstrated. This electrode was used to monitor photosynthetic sulfide oxidation by Chlorobium phaeobacteroides. The electrode was resistant to Pfennig's medium and bacterial attack and after five months of use showed a potential drift within ±2 mV corresponding to ±13% maximum. The results obtained in a batch type experiment of growth of Chlorobium at different pH values suggest that Chlorobium cells preferentially take up the HS− ion rather than H2S. |
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Keywords: | glass/silver sulfide electrode pH2S photosynthetic bacteria Chlorobium phaeobacteroides |
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