Solid lipid nanoparticles of anticancer drug andrographolide: formulation,in vitro and in vivo studies |
| |
Authors: | Rabea Parveen F J Ahmad Z Iqbal M Samim |
| |
Affiliation: | 1. Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard
New DelhiIndia;2. Department of Chemistry, Faculty of Science, Jamia Hamdard
New DelhiIndia |
| |
Abstract: | Diterpenoidal anti-cancer drug andrographolide (AD) was encapsulated into solid lipid nanoparticle (SLN) because of poor aqueous solubility and high lipophilicity. AD-SLNs were prepared by solvent injection method and characterized for droplet size, surface morphology, zeta potential, etc. In vitro drug release was carried out by dialysis-membrane method. A pharmacokinetic study was performed by UPLC/Q-TOF-MS method to determine the maximum plasma concentration (Cmax), area under the curve (AUC), etc. There was an improvement in Cmax and AUC of AD-SLNs when compared with AD, thereby enhancing the bioavailability of AD. The tmax was increased than that of AD suspension, indicating the sustained release pattern of AD-SLNs. The antitumor activity was carried out on Balb/c mice showing better results with AD-SLNs as compared to AD. Thus, the AD-loaded SLNs would be useful for delivering poorly water-soluble AD with enhanced bioavailability and improved antitumor activity. |
| |
Keywords: | Andrographolide antitumor activity pharmacokinetics solid lipid nanoparticles UPLC/Q-TOF-MS method |
|
|