Synthesis and evaluation of ciprofloxacin-loaded carboxymethyl tamarind kernel polysaccharide nanoparticles |
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Authors: | Neeraj Dilbaghi Harmanmeet Kaur Munish Ahuja Pooja Arora Sandeep Kumar |
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Affiliation: | 1. Nanomaterials Synthesis &2. Characterization Laboratory, Department of Bio &3. Nanotechnology , Guru Jambheshwar University of Science &4. Technology , Hisar , Haryana , 125001 , India;5. Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science & |
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Abstract: | Nanotechnology is currently employed as a tool to fight more efficiently against human pathogens. Nanoparticles can be prepared from a variety of materials such as protein, biodegradable polymers and synthetic polymers. Tamarindus indica Linn. or tamarind is one of the most important biodegradable polymer. In the present study, chemically modified polymer of tamarind ‘carboxymethyl tamarind kernel polysaccharide’ (CMTKP) is used for the synthesis of nanoparticulate formulation. Antibacterial activity of CMTKP was analysed which was then enhanced by incorporating a flouroquinolone antibiotic, ciprofloxacin to it. Ciprofloxacin-loaded CMTKP nanoparticles were synthesised via ionotropic gelation technique. Nanosuspension so formed was lyophilised by addition of a cryoprotectant. Nanoparticles obtained were characterised for its particle size, morphology and stability. Interaction studies were confirmed by Fourier transform infrared spectroscopy (FT-IR). Antibacterial activities of ciprofloxacin, CMTKP and ciprofloxacin-loaded CMTKP nanoparticles were tested against two Gram negative and positive bacteria. The antibacterial assay results revealed greatest zone of inhibition by ciprofloxacin-loaded CMTKP nanoparticles in Micrococcus luteus. Toxicity analysis of the prepared formulation was carried out on vero cell lines via resazurin assay which revealed its minimum toxicity. |
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Keywords: | ciprofloxacin carboxymethyl tamarind kernel polysaccharide antibacterial ionotropic gelation nanoparticles |
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