| 固相萃取精制苦瓜皂苷工艺研究 |
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| 引用本文： | 李健,刘鑫,刘宁,陈姝娟,黄艳.固相萃取精制苦瓜皂苷工艺研究[J].化学世界,2009,50(8). |
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| 作者姓名： | 李健 刘鑫 刘宁 陈姝娟 黄艳 |
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| 作者单位： | 哈尔滨商业大学食品工程学院,黑龙江,哈尔滨,150076 |
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| 基金项目： | 黑龙江省自然科学基金重点项目 |
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| 摘 要： | 以苦瓜为原料,建立了SPE精制、HPLC检测、ESI-MS定性为主要步骤的苦瓜皂苷的分析方法。通过HPLC测定,得到了SPE精制的最佳工艺条件为:上样pH为8,经10%、20%乙醇各15mL淋洗后用10mL40%乙醇淋洗,洗脱液为65%乙醇,洗脱体积为7mL,流速为1mL/min,此条件下所得液相色谱的峰数、峰形、峰面积均较理想。测得最佳条件下精制的苦瓜总皂苷含量为82.13%。经ESI-MS定性分析得出,精制后的产物为三萜类皂苷。苦瓜皂苷快速分离方法的研究,对其构效关系和生物活性研究提供了重要的前提条件。
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| 关 键 词： | 苦瓜皂苷 固相萃取(SPE) 高效液相色谱(HPLC) |
Study on the Purification Technology of Momordica Saponins by Solid-phase Extraction |
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| LI Jian,LIU Xin,LIU Ning,CHEN Shu-juan,HUANG Yan.Study on the Purification Technology of Momordica Saponins by Solid-phase Extraction[J].Chemical World,2009,50(8). |
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| Authors: | LI Jian LIU Xin LIU Ning CHEN Shu-juan HUANG Yan |
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| Affiliation: | Institute of Food Sciences and Engineering;Harbin University of Commerce;Heilongjiang Harbin 150076;China |
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| Abstract: | The analysis method of momordica saponins with SPE purification,HPLC detection and ESI-MS qualitative analysis was established using momordica as raw material.The optimum conditions of SPE purification were determined by HPLC,and the results were as follows:the sample pH value was 8;it was leached with 10 mL 40% ethanol after washing with 10% and 20% ethanol;the elution solution was 7 mL 65% ethanol;and the flow rate was 1 mL/min.On this condition,the peak numbers,peak shape and peak areas of the HPLC profi... |
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| Keywords: | momordica saponins solid-phase extraction HPLC |
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