| DPPH法测定新疆磨合烟的抗氧化活性 |
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| 引用本文： | 倪国柱,温琳豫,王健,朱萍萍,胡曙晨,支玲.DPPH法测定新疆磨合烟的抗氧化活性[J].广州化工,2012,40(22):104-106. |
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| 作者姓名： | 倪国柱 温琳豫 王健 朱萍萍 胡曙晨 支玲 |
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| 作者单位： | 新疆医科大学药学院,新疆乌鲁木齐,830011 |
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| 基金项目： | 新疆医科大学第六期大学生创新性实验计划项目“薄层色谱生物自显影追踪分离新疆烟草中天然乙酰胆碱酯酶抑制剂的研究” |
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| 摘 要： | 利用紫外分光光度仪,通过DPPH方法对新疆磨合烟提取物的抗氧化活性进行了研究。以Vc为对照,IC50值作为评价试样清除自由基能力为指标,建立了新疆磨合烟提取物抗氧化活性的测定方法,结果表明：通过与Vc对自由基的清除率进行比较,经酸提回流法处理的新疆磨合烟具有较好的抗氧化活性,该实验为新疆磨合烟的应用及抗乙酰胆碱酯酶的药理作用的进一步研究提供了依据。
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| 关 键 词： | 新疆莫合烟 DPPH法 抗氧化活性 |
The Research of Antioxidant Activity of Xinjiang Mohe Tobacco with DPPH Method |
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| NI Guo-zhu,WEN Lin-yu,WANG Jian,ZHU Ping-ping,HU Shu-chen,ZHI Ling.The Research of Antioxidant Activity of Xinjiang Mohe Tobacco with DPPH Method[J].GuangZhou Chemical Industry and Technology,2012,40(22):104-106. |
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| Authors: | NI Guo-zhu WEN Lin-yu WANG Jian ZHU Ping-ping HU Shu-chen ZHI Ling |
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| Affiliation: | (College of Pharmacy,Xinjiang Medical University of China,Xinjiang Urumqi 830011,China) |
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| Abstract: | The antioxidant activity of Xinjiang Mohe Tobacco was studied with DPPH method in UV spectrophotometer.The method in scavenging free radicals was established through evaluating the ability for scavenging free radicals of the extract of Xinjiang Mohe Tobacco with Vc in control while IC50 as index.Though the comparison of the ability for scavenging free radicals between the extract of Xinjiang Mohe Tobacco and Vc,the extract through acid solution reflux was more stronger.The experiment may provide basis for the further investigation in pharmacological effects of anti-acetylcholinesterase and application of Xinjiang Mohe Tobacco. |
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| Keywords: | Xinjiang Mohe Tobacco DPPH method antioxidant activity |
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