新疆低温稠油油藏厌氧硝酸盐还原菌与硫酸盐还原菌富集产物菌群分析

基于16S rDNA分子克隆文库方法,分析新疆克拉玛依油田六中区采油井T6191井口样品硝酸盐还原菌(NRB)和硫酸盐还原菌(SRB)富集产物的菌群多样性。通过核糖体DNA扩增片段的限制性酶切片段长度多态性分析(ARDRA)将NRB和SRB文库中的克隆分为11个和12个系统发育类型。NRB克隆文库中主要微生物菌群为施氏假单胞菌(Pseudomonas stutzeri)(35%)、未培养拟杆菌(Uncultured Bacteroidetes bacterium)(37%)、螺旋体(Spirochaeta)(9%)和产氨基酸杆菌(Acidaminobacter hydrogeno formans)(9%);SRB克隆文库中主要微生物菌群为脱硫弧菌(Des-ulfovibrio caledoniensis)(43%)、未培养拟杆菌(20%)、脱硫单胞菌(Desulfuromonas michiganensis)(12%)和螺旋体(8%)。NRB菌群与SRB菌群结构虽然存在差异,但未培养拟杆菌和螺旋体在两种富集产物中均出现,特别是未培养拟杆菌在2个文库中都占有一定优势地位。为油田开发NRB抑制硫酸盐还原作用新技术提供了理论依据。

Anaerobic Microbial Community in Nitrate Reducing Bacteria and Sulfate Reducing Bacteria Enrichment Culture of Low Temperature Heavy Oil Reservoir in Xinjiang

16S rRNA gene clone library construction was used to analyze the microbial communities of NRB and SRB enrichment culture inoculated with oil-water samples from the Middle Block No.6 of Karamay Oil Field in Xinjiang.Clone libraries NRB and SRB were identified 11 and 12 taxanomic operational units(OTUs),respectively,by amplified ribosomal DNA restriction analysis(ARDRA).Sequence analysis of the representative clone of each OTU showed that dominant bacteria of NRB were affiliated with Pseudomonas stutzeri(35%),uncultured Bacteroidetes bacterium(37%),Spirochaeta(9%) Acidaminobacter hydrogenoformans(9%);dominant bacteria of SRB were Desulfovibrio caledoniensis(43%),uncultured Bacteroidetes bacterium(20%) and Desulfuromonas michiganensis(12%),Spirochaeta(8%).There was great difference in microbial populations among the enrichment cultures of NRB and SRB,however,uncultured Bacteroidetes bacterium and Spirochaeta appeared in both enrichment cultures.Especially,uncultured Bacteroidetes bacterium occupied dominant status.It provides foundation for the application of NRB controlling the activity of SRB.