| 降解秸秆的纤维素酶产生菌的筛选及产酶条件研究 |
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| 引用本文： | 武峥,张迎君,周心智.降解秸秆的纤维素酶产生菌的筛选及产酶条件研究[J].纤维素科学与技术,2009,17(2):20-26. |
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| 作者姓名： | 武峥 张迎君 周心智 |
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| 作者单位： | 重庆市农业科学院果树研究所,重庆,江津,402260 |
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| 基金项目： | 重庆市科委自然科学基金 |
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| 摘 要： | 从土壤、霉变的农产品和实验室保存的真菌中筛选到15株产纤维素酶的菌株,其中绿色木霉T206产酶能力最强。利用液体发酵,研究了碳源、氮源、接种量、起始pH值、培养时间等对菌株产酶的影响,以及该菌株所产纤维素酶的种类。在最适条件下菌株培养96h后,羧甲基纤维素酶活(CMCA)最高达到7654.33U,是优化前酶活的1.7倍,滤纸酶活(FPA)达到1675.12U。
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| 关 键 词： | 绿色木霉 纤维素酶 筛选 产酶条件 |
Screening of Cellulase Producing Strain for Straw Degradation and Optimization of Fermentation |
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| WU Zheng,ZHANG Ying-jun,ZHOU Xin-zhi.Screening of Cellulase Producing Strain for Straw Degradation and Optimization of Fermentation[J].Journal of Cellulose Science and Technology,2009,17(2):20-26. |
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| Authors: | WU Zheng ZHANG Ying-jun ZHOU Xin-zhi |
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| Affiliation: | (Fruit Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing 402260, China ) |
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| Abstract: | 15 strains with performance to hydrolyze cellulose were screened from soil, rotten produce and preserved fungi in our laboratory. A Trichoderma Viride T206 with relatively high cellulase activity was isolated. The isolated sample was fermented in liquid state after isolation, and its fermentation conditions （carbon source, nitrogen source, inoculating volume, initial pH and cultivating time） and enzyme component were studied. Under optimum condition, its hydrolysable efficiency upon carboxymethyl cellulose and filter paper reached 7654.33 U and 1675.12 U. |
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| Keywords: | Trichoderma Vride eellulase screening fermentation |
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