| 穿山龙薯蓣皂苷酶解产物的分离纯化 |
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| 引用本文： | 刘冰,王元好,朱宏丽,鱼红闪,金凤燮.穿山龙薯蓣皂苷酶解产物的分离纯化[J].大连工业大学学报,2006,25(3). |
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| 作者姓名： | 刘冰 王元好 朱宏丽 鱼红闪 金凤燮 |
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| 作者单位： | 大连轻工业学院,生物与食品工程学院,辽宁,大连,116034;大连轻工业学院,生物与食品工程学院,辽宁,大连,116034;大连轻工业学院,生物与食品工程学院,辽宁,大连,116034;大连轻工业学院,生物与食品工程学院,辽宁,大连,116034;大连轻工业学院,生物与食品工程学院,辽宁,大连,116034 |
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| 摘 要： | 用柱层析法分离纯化穿山龙薯蓣皂苷的酶解产物,硅胶柱中的流动相为氯仿与甲醇的不同比例,经硅胶柱分离得到薯蓣皂苷酶解产物的两种单体,为产物Ⅰ和产物Ⅱ,用薄层层析法和高效液相色谱法检测,得到产物Ⅰ为3-(O)-(-βD-吡喃葡萄糖基)-薯蓣皂苷元,得率为17.85%,纯度为69.41%。同时得到产物Ⅱ,得率为1.64%。产物2为反应副产物,其结构、性质及药理作用有待于进一步研究。
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| 关 键 词： | 薯蓣皂苷 酶解产物 分离 纯化 |
Separation and purification of dioscin hydrolysate |
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| Liu Bing,WANG Yuan-hao,Zhu Hong-li,YU Hong-shan,JIN Feng-xie.Separation and purification of dioscin hydrolysate[J].Journal of Dalian Dalian Polytechnic University,2006,25(3). |
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| Authors: | Liu Bing WANG Yuan-hao Zhu Hong-li YU Hong-shan JIN Feng-xie |
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| Abstract: | Two kinds of dioscin hydrolysates were separated and purified by silica gel column gradient elution with chloroform and methanol as solvent.The hydrolysate Ⅰ was identified to be 3-(O)-(β-D-glucopyranoside)-diosgenin by TLC and HPLC.The yield of the purified dioscin from enzyme reaction was 17.85%.The purity of 3-(O)-(β-D-glucopyranoside)-diosgenin was detected to be about(69.41%.)The hydrolysate Ⅱ is an outgrowth with 1.64% of yield.Its structure, property,pharmacology functions are yet to be investigated. |
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| Keywords: | dioscin hydrolysate separation purification |
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