Method for identification of mutant glutathione S-transferases conferring enhanced resistance to the anti-cancer drug chlorambucil |
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| Authors: | Hirota Kiyonori Hanyu Yoshiro |
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| Affiliation: | Molecular and Cell Biology, National Institute ofAdvanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. |
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| Abstract: | We screened library of mutant glutathione S-transferases (GSTs) in Escherichia coli by successive treatments with anti-cancer drug chlorambucil and identified mutant GSTs that conferred enhanced resistance to host against chlorambucil compared with wild-type GST. This study provides a method to develop enzymes with improved efficiency of detoxification against cytotoxic substances. |
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| Keywords: | anti-cancer drug chlorambucil glutathione S-transferase detoxification mutant drug resistance |
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